Journal: Immunity

Article Title: Vaccination Induces Maturation in a Mouse Model of Diverse Unmutated VRC01-Class Precursors to HIV-Neutralizing Antibodies with >50% Breadth

doi: 10.1016/j.immuni.2020.12.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies with less than 10% breadth were excluded from this analysis. . Bio-layer interferometry antigenicity analysis Kinetics and affinities of antibody-antigen interactions were measured on an Octet (Forte Bio) using Anti-human IgG Fc and Anti- mouse IgG Fc capture biosensors following the instructions.

Techniques: Staining, Sequencing, Expressing, Plasmid Preparation, Software

Journal: Immunity

Article Title: Vaccination Induces Maturation in a Mouse Model of Diverse Unmutated VRC01-Class Precursors to HIV-Neutralizing Antibodies with >50% Breadth

doi: 10.1016/j.immuni.2020.12.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies with less than 10% breadth were excluded from this analysis. . Bio-layer interferometry antigenicity analysis Kinetics and affinities of antibody-antigen interactions were measured on an Octet (Forte Bio) using Anti-human IgG Fc and Anti- mouse IgG Fc capture biosensors following the instructions.

Techniques: Staining, Sequencing, Expressing, Plasmid Preparation, Software

Journal: bioRxiv

Article Title: Reprogramming BCMA-Targeted CAR-T Cells through γ-Secretase Modulation Blocks Antigen Shedding and Extends CAR-T Longevity

doi: 10.64898/2026.01.20.700575

Figure Lengend Snippet: (A) Structural modeling of murine 4C8A clone versus LLM-based humanized scFv variants showing preserved CDR orientation toward BCMA and specificity for the distinct epitope. (B) RMSD plots for the snapshots from MD simulation of the scFvs (murine and humanized) in complex with BCMA TM with three technical replicates. (C) MM/GBSA based free energy binding plots for the murine and humanized scFv-BCMA complexes. (D) Workflow of immunogenicity assay showing antibody-primed dendritic cells co-cultured with autologous PBMCs. (E-G) Cytokine analysis of IFN-γ, IL-2, and IL-4 showing lower levels for humanized CARs versus murine. Fully human IgG antibody was used as reference control. (H) Representative super-resolution imaging shows uniform membrane localization of CAR constructs (magenta). Nuclei were stained with DAPI (blue). Corresponding bar graph of the image analysis (n=6). (I1) Dot plots of flow cytometry quantification of CAR surface expression using GS-linker antibody (I2) Bar graph of the flow cytometry data showing percentage CAR-Transduction (n=5). (J1) Cell-based binding assay using flow cytometry showing affinity gain for the CDR-optimized humanized CAR (HmzCAR) (J2) Bar graph of the analysis (n=5). (K) Workflow of BLI sensorgrams and kinetic analysis. (L1, L2) Bio-layer interferometry (BLI) sensogram showing real-time binding kinetics of the indicated analytes. Colored traces represent different concentrations, with an initial association phase followed by dissociation. (M) Experimental workflow of co-culture of CAR-T cells with BCMA expressing target cells. (N) Cytotoxicity against MM.1S cells across different E:T ratios (O) Similarly, for MM.1R cells (n=5). (P) Representative flow cytometry contour plots of granzyme-B secretion (P2) Mean fluorescence intensity (MFI) of the flow cytometry contour plots. Data represent mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001. A non-parametric t-test was used for statistical analysis between groups.

Article Snippet: Kinetic binding analyses between BCMA Antigen and the designed ScFvs were conducted using the Gator Biolayer Interferometry (BLI) system (Gator Bio Inc., USA) at room temperature.

Techniques: Binding Assay, Immunopeptidomics, Cell Culture, Control, Imaging, Membrane, Construct, Staining, Flow Cytometry, Expressing, Transduction, Cell Binding Assay, Co-Culture Assay, Fluorescence